ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of maxillary sinus elevation with gene-enhanced tissue engineering bone in dogs.</p><p><b>METHODS</b>bone marrow stromal cells (BMSC) derived from dog marrow were cultured, and transduced with the adenovirus carrying bone morphogenetic protein-2 (BMP-2) gene (AdBMP-2), the adenovirus carrying green fluorescent protein (GFP) gene (AdGFP) in vitro. The bone formation ability of gene modified BMSC with scaffold was examined in nude mice and in elevated maxillary sinus of dog. Student's t-test was used for statistical analysis with SPSS 11.0 software package.</p><p><b>RESULTS</b>Gene transfection efficiency reached up to (83.95 ± 2.43)% as demonstrated by GFP expression. Ectopic bone formation was detected in nude mice. As for maxillary sinus floor elevation in a dog model, new bone formation area in the AdBMP-2 gene transduced BMSC with Bio-Oss group was significantly higher than in BMSC with Bio-Oss group at 120 d (P < 0.05).</p><p><b>CONCLUSIONS</b>AdBMP-2 gene transduced BMSC can stimulate ectopic bone formation in nude mice, and promote bone formation and maturation in the dog maxillary sinus.</p>
Subject(s)
Animals , Dogs , Male , Mice , Adenoviridae , Genetics , Bone Matrix , Transplantation , Bone Morphogenetic Protein 2 , Genetics , Metabolism , Bone Transplantation , Methods , Cells, Cultured , Mesenchymal Stem Cells , Cell Biology , Metabolism , Mice, Nude , Minerals , Osteogenesis , Recombinant Proteins , Genetics , Metabolism , Sinus Floor Augmentation , Methods , Tissue Engineering , Methods , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of tissue engineering bone in maxillary sinus lifting.</p><p><b>METHODS</b>The marrow stromal stem cells of dog were cultured in DMEM containing 100 m1/L fetal bovine serum and induced to differentiate to osteoblasts, which were then inoculated together with Bio-Oss for 5 days. Sixteen dog's bilateral maxillary sinus were elevated. One side was grafted with a compound of BMSC and Bio-Oss and the other side grafted with Bio-Oss alone. The samples were studied by gross, CT, histomorphology and histomorphometrical analysis at the 30th, 90th day after the operation. t-test was used for statistical analysis.</p><p><b>RESULTS</b>In gross view and CT, new bone formation was observed in all maxillary sinus after 30 and 90 days respectively. Histomorphometrical analysis showed much more new callus in BMSC-Bio-Oss group than in Bio-Oss group (P < 0.05).</p><p><b>CONCLUSIONS</b>A better effect of new bone formation could be obtained with tissue engineered bone in maxillary sinus lifting.</p>